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While classical microbiological techniques prove their limits to study organisms in their environments (only few percent of living organisms are prone to cultivation), molecular biology tools prescinding from these limitations allowed to extend our knowledge about these organisms : who are they, how do they live and interact. Mostly, DNA and RNA sequencing techniques progresses, which showed impreceded efficiency gains in the last 15 years, were the main tools of this revolution.
This communication will discuss these changes, with examples covering several environments, from waste water plants, soil, human microbiome to oceans. In a prospective conclusion, impacts of most recent single molecule sequencing technologies in this field will be discussed.
Biography : After completing a PhD in immunology (Université Pierre et Marie Curie & Institut Pasteur, Paris), Eric Pelletier became researcher at CEA / Genoscope. He’s focusing on genomics data analysis nd using HTC/HPC methods. After having been involved in the Human Genome Project, he specialized since 2001 in environmental genomic of prokaryotes (waste water plants, soils, intestinal tract). He participate in the MetaHit project that produced the first bacterial gene catalogue from the human gut. He’s now working on the marine eukaryote plankton diversity in the Tara oceans project, and just published the first gene catalogue of these organisms. |
Jean-Pascal JEGU
Tel : +33 (0)9 70 65 02 10
jean-pascal.jegu@teratec.fr
Campus TERATEC
2, rue de la Piquetterie
91680 BRUYERES-LE-CHATEL
France